Excitation wavelength: The incoming light uses this wavelength of light to illuminate the fluorescent dye/sample and “activate” it.
Emission wavelength: After the emitted light sample is activated, it re-emits its own fluorescence, which is this wavelength.
Think of fluorescent molecules as small rechargeable bulbs:
· Excitation wavelength = charging voltage/power supply (give it energy)
· Emission wavelength = light emitted by yourself after the light bulb is lit
The emission wavelength must be longer than the excitation wavelength, that is: excitation light polarization short wave, the emission light polarization wave energy is consumed a little, the light wavelength is longer, the color is more reddish.
· Excitation: 358nm (ultraviolet light, shining in)
· Emission: 461nm (blue fluorescence, emitted)
· Excitation: 488nm (blue light exposure)
· Emission: 525nm (green light emission)
4.1. The light source emits excitation wavelength → light to the specimen
4.2. Specimen fluorescent molecules are excited to → emit fluorescence at emission wavelengths
4.3. The microscope filter filters out the harsh excitation light, leaving only fluorescence, and we can see a clear fluorescence image
Tell us the material, the feature you need to see and the magnification range. We will recommend a configuration and quote it.
